Hexose Transport in Isolated Brown Fat Cells
نویسندگان
چکیده
منابع مشابه
Activation of hexose transport by concanavalin A in isolated brown fat cells. Effects of cell surface modification with neuraminidase and trypsin on lectin and insulin action.
Direct activation of the fat cell n-glucose transport system by the lectin concanavalin A was studied using a rapid filtration procedure which monitors 3-O-[3H]methylglucose uptake in isolated brown fat cells. Transport of this nonmetabolized D-glucose analogue, which occurs by facilitated diffusion, was linear for 5 or more min, temperature-dependent, and inhibited by unlabeled n-glucose, phlo...
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The co-transport mechanism of the coupled movement of D-glucose and Na+ across the brush-border membrane is now universally accepted (for a review, see Crane, 1977). The transport agency shows a stable asymmetry with respect to the plane of the membrane, as demonstrated by its asymmetric inactivation (or reactivation) by little-permeant or impermeant reagents. Comparison was made between little...
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The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional glucose transport inhibitors such as phloretin, phlorizin and cytochalasin B but n...
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The pattern of L-alanine uptake in isolated cells of interscapular brown adipose tissue has been determined. The uptake can be divided into the diffusion component (Kd = 0.55 min-1) and a saturable Na(+)-dependent transport (KM = 0.87 mM and Vmax = 155 nmol/min/10(6) cells). The saturable component can be subdivided into MeAIB-sensitive (KM = 1.63 mM and Vmax = 162 nmol/min/10(6) cells) and MeA...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)79744-9